k7m2 cells  (ATCC)

Journal: bioRxiv

Article Title: Collagen targeting IL-12 combined with Doxorubicin enhances the anti-tumor effect against osteosarcoma

doi: 10.64898/2026.05.07.723520

Figure Lengend Snippet: The box plot of (a) collagen type I and (b) collagen type III expression in OS tissue compared with paired normal bone samples. (c) Collagen type I and III expression in both mouse (BVM03O and K7M2) and human OS. The cryosections were imaged by confocal microscopy. Nucleus and collagen type I or collagen type III were blue and red, respectively. Scale bars, 250 μm. The results are expressed as mean ± S.E.M. Statistical analysis was performed using an unpaired t-test for (a) and (b). *P < 0.05, **P < 0.01. N.S., not significant; PBS, phosphate-buffered saline.

Article Snippet: K7M2 cells (CRL-2836) were purchased from ATCC.

Techniques: Expressing, Confocal Microscopy, Saline

Journal: bioRxiv

Article Title: Collagen targeting IL-12 combined with Doxorubicin enhances the anti-tumor effect against osteosarcoma

doi: 10.64898/2026.05.07.723520

Figure Lengend Snippet: We inoculated mice with 3 × 10 6 BVM03O OS cells subcutaneously on the back skin and the mice were treated with PBS (i.v., n = 5), 416.6 pmol of CBD–IL-12 (i.v., n = 5), and 416.6 pmol of CBD–IL-12 + 2 mg/kg of Dox (i.v., n = 5) on day 35. Dox was additionally injected 3 and 6 days post initial treatment. Average tumor growth curve (a), individual tumor curves (b), and survival curves (c) are shown. We inoculated mice with 5 × 10 6 K7M2 OS cells subcutaneously on the back skin and the mice were treated with PBS (i.v., n = 4) and 416.6 pmol of CBD-IL-12 + 2 mg/kg of Dox (i.v., n = 3) once the tumor volume reaches 50 mm 3 . Dox was additionally injected on day 3 and day 6. Average tumor curve (d), individual tumor curves (e), and survival curves (f) are shown. The results are expressed as mean ± S.E.M. Log-rank test was used for (c) and (f) in the statistical analysis. *P < 0.05, **P < 0.01. N.S., not significant; PBS, phosphate-buffered saline.

Article Snippet: K7M2 cells (CRL-2836) were purchased from ATCC.

Techniques: Injection, Saline

Journal: Advanced Science

Article Title: Osteosarcoma Cell‐Derived Migrasomes Promote Macrophage M2 Polarization to Aggravate Osteosarcoma Proliferation and Metastasis

doi: 10.1002/advs.202409870

Figure Lengend Snippet: MANPs promote macrophage phagocytosis and M2 polarization via Milk fat globule‐EGF factor 8 (MFGE8). A‐H) 4D proteome sequencing analysis of cell body, migrasome and MANP. (A‐D) Heatmaps and volcano plots of DEGs in protein‑seq analysis of cell body, migrasome and MANP ( n = 3). (E, F) Heatmaps of TSPAN proteins, integrin proteins, and histones in cell body, migrasome and MANP ( n = 3). (G) Venn diagram showing proteins co‐enriched by migrasomes and MANPs. (H) Relative expression of MFGE8 in cell body, migrasome and MANP ( n = 3). I) Immunostaining images of WGA (green) and MFGE8 (red) in OS cells ( n = 3). Magnified images of migrasomes and MANPs are shown on the right. Low magnification: scale bars = 10 µm; high magnification: scale bars = 1 µm. J,K) qRT‐PCR and WB analysis of MFGE8 knockdown efficiency in K7M2 wt cells ( n = 3). L,M) WB analysis of MFGE8 knockdown efficiency in migrasomes and MANPs ( n = 3). N‐Q) BMDMs treated with 10 µg mL −1 sh‐NC MANPs or sh‐MFGE8 MANPs for 24 h. (N, O) Representative immunostaining images and quantitative analysis of BMDMs (red) phagocytosis of microbeads (green) ( n = 3). Low magnification: scale bars = 100 µm; high magnification: scale bars = 50 µm. (P, Q) Flow cytometry analysis of the proportion of BMDMs (APC) that phagocytose apoptotic OS cells (CFSE) and quantitative analysis ( n = 3). R‐T) BMDMs treated with 10 µg mL −1 sh‐NC MANPs or sh‐MFGE8 MANPs for 24 h, followed by co‐incubation with apoptotic OS cells for 24 h. (R) qRT‐PCR analysis of the expression of M1 polarization and M2 polarization markers in BMDMs ( n = 3). (S, T) Flow cytometry analysis of the proportion of M2‐type macrophages and quantitative analysis ( n = 3). Results were shown as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. One‐way ANOVA test or two‐way ANOVA test was used for multivariate analysis. Unpaired t‐tests were used for the comparison of two groups.

Article Snippet: The human OS cell lines 143b and MG63 and the mouse OS cell line K7M2 wt were obtained from the American Type Culture Collection (ATCC), and the OS cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM, C11995500BT, Gibco, USA) supplemented with 1% penicillin/streptomycin (P/S, 15140122, Gibco, USA) and 10% fetal bovine serum (FBS, 10091148, Gibco, USA).

Techniques: Sequencing, Expressing, Immunostaining, Quantitative RT-PCR, Knockdown, Flow Cytometry, Incubation, Comparison